STUDY ON THE PROPAGATION OF TRIPLOID ACACIA HYBRID CLONES X201 AND X205 BY TISSUE CULTURE

Abstract

In the last decade, breeding on polyploidy has been conducted for tropical acacias. Some new triploid acacia hybrid clones with fast-growing and longer fibre lengths were selected and recognized as new forest cultivars. The study on vegetative propagation by tissue culture, therefore is required for better deployment. The results showed that using HgCl₂ 0.1% to sterile shoots in 10 mins has the highest adventitious shoot rates (58.5 - 65.4%). The modified Murashige and Skoog media (MS*) with phytohormones were evaluated for their suitability to support shoot induction, shoot multiplication and root formation. The highest percentage of multiple shoot induction was achieved on MS + BAP (1.5 mg/l). Elongated shoots showed the best rooting response on 1/2 MS* + IBA (2.0 mg/l) with rooted shoot rates reached up to 97%.