In vitro propagation of Pinus caribaea Morelet from seeds of plus tree family
Keywords:
Pinus caribaea Morelet,, plus tree, in vitro propagation, cuttings,, , seedlingsAbstract
Pinus caribaea Morelet has potential for afforestation in Vietnam because
of its fast growing ability, good wood properties, adaptation to different site
conditions, and good resistance to wind and storm. However, the
propagation by seeds or cuttings has not met the needs of afforestation.
Therefore, in vitro propagation of P. caribaea from dominant seed plots
is an effective way to develop varieties with improved yield and quality
into production. In this study, we carried out an in vitro propagation for
P. caribaea using seeds obtained from selected plus trees. P. caribaea
seeds were germinated on cotton wool. The stem segments taken from the
40 - day-old seedlings were used in the sterilization process. The best
sterilization result was using 0.1% HgCl2 for 12 minutes. The optimal shoot
multiplication medium was MS* + 0.25mg/l BAP + 4.25g/l agar + 30g/l
sugar. The cycle of shoot multiplication was from 40 - 45 days, after that,
there was a decrease in the number of shoots and a restriction in height
growth in bud clusters. The most suitable medium for in vitro rooting was
1/2 MS* + 1mg/l IBA + 4.5g/l agar + 30g/l sugar. In vitro shoots began to
root after 4 weeks and completed rooting after 6 weeks.
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