Evaluation of the genetic diversity and population structure of Pinus caribaea in Vietnam
Keywords:
Genetic diversity,, Pinus caribaea, population structure, variantsAbstract
Pinus caribaea Morelet is divided into 3 variants hondurensis, bahamensis and caribaea based on the species magnetic distribution in different geographic regions. The results showed that 58 DNA segments were accounted with polymorphic segments accounting for 92.16%, mean genetic diversity coefficient h = 0.329; mean Shannon coefficient I = 0.491, and average polymorphic fraction ratio PPB = 92.16%. The highest genetic diversity belongs to the var. hondurensis (I = 0.542; h = 0.364 and PPB = 100%) and the lowest is the var. bahamensis (I = 0.453; h = 0.308 and PPB = 79.41%). The GST genetic differentiation index is only 0.0932, proving that the level of genetic difference between variants is relatively low at only 9.32% (< 10%). The calculated gene exchange index (Nm) value of 4.8670 indicates that the frequency of gene exchange between variants is relatively high. Analysis of the level of molecular change between 3 variants, the species showed that the level of molecular change between 3 strains was low at only 9% and between families within the same strain was high (reaching 91%) with a p-value of < 0.001. The genetic distance ranged from 0.034 to 0.113 and the degree of similarity ranged from 0.894 (84.9%) to 0.967 (96.7%). In the genetic relationship at species level, the var. hondorensis and var. bahanensis have a closer genetic relationship with each other than the var. caribaea
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