Evaluation of genetic diversity and relationship of introduced Sonneratia apetela Buch - Ham provenances in Vietnam using ISSR markers
Keywords:
DNA markers, genetic diversity,, ISSR,, Sonneratia apetala Buch - HamAbstract
Sonneratia apetela Buch - Ham has been planted in some mangrove
ecosystems in Nam Dinh and Thai Binh provinces. Despite of being widely
used in afforestation in these areas, there is no baseline of genetic information
of this species regarding to genetic diversity. The use of molecular markers to
assess genetic diversity among S. apetela provenances will, therefore, provide
the essential information for breeding and deployment program for this
species. In this study, 8 ISSR markers were used to evaluate the genetic
diversity and relationship between 6 provenances of S. apetala, which were
imported and planted in Nam Dinh and Thai Binh. A total of 87 DNA
fragments were detected, in which 63 were polymorphic (72.61%). The result
showed the high level of genetic diversity of studied samples (h = 0.257). The
genetic similarity coefficient among provenances ranged from 0.892 to 0.966.
The phylogeny of S. apetela were divided into 2 branches, the first one has
only provenance from Myanmar in 2003 and the other consisted of 5 remained
provenances (Hainan, Guangdong, Myanmar in 1995, Tanintharyi - Myanmar,
Ayeyarwady - Myanmar). Some recommendations for breeding and deployment
programs were also addressed.
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