Genetic diversity of the populations of Michelia tonkinensis A. Chev. in some North provinces, Vietnam using SSR marke
Keywords:
Genetic diversity,, Michelia tonkinensis, SSRAbstract
Michelia tonkinensis A.Chev. is a multi-purpose timber species, with high
economic and conservation value. However, the populations of M.
tonkinensis in natural forests are reduced due to over exploitation. In this
study, 25 SSR markers were used to analyze the genetic diversity of 50
dominant individuals M. tonkinensis collected in 5 provinces Hoa Binh,
Thanh Hoa, Phu Tho, Lao Cai, Lai Chau. Among them, the level of genetic
diversity in Hoa Binh population was the highest (Na = 3.920; Ne = 2.588; I
= 0.966; Ho = 0.561; He = 0.515 and PPB = 96%) and the lowest in Thanh
Hoa (Na = 2.200; Ne = 1.984; I = 0.600; Ho = 0.560; He = 0.372 and PPB =
72%). All 5 populations had the private allele (mean of Ap = 0.360) and crosspollination. The gene flow (Nm) has also occurred in the M. tonkinensis
populations with an average value of Nm = 1.884. The total level of
molecular variance (AMOVA) was relatively low among populations
(37.07%) and high among individuals within the populations (62.93%). The genetic similarity of M. tonkinensis populations ranged from 58 to 90%.
Molecular analysis results showed that M. tonkinensis populations had
relatively high genetic diversity. The cross-pollination also occurred among
individuals in the population and among the populations, so that ensure the
maintenance of genetic diversity in population the next generation.
Therefore, the M. tonkinensis populations should be protected at the
individual level, serving the selection and replication of species.
References
1. Boys J., Cherry M., Dayanandan S., 2005. Microsatellite analysis reveals genetically distinct populations on red pine (Pinus resinosa, pinaceae). Amer. J. Bot. 92(5): 833-841.
2. Echt C.S., May-Marquardt P., Hseih M., Zahorchak R., 1996. Characterization of microsatellite markers in eastern white pine. Genome 39: 1102-1108.
3. Elsik C.G., Minihan V.T., Hall S.E., Scarpa A.M., Williams C.G., 2000. Low-copy microsatellite markers for Pinus taeda L. Genome 43: 550-555.
4. Goudet J., 1995. FSTAT version 1.2: a computer program to calculate F-statistics. J. Hered. 86: 485-486.
5. Hoang Van Sam, 2008. Uses and conservation of plant species in a national park - A case study of Ben En National Park, Vietnam. Economic Botany 62(4): 574 -593.
6. Hung K.H., Lin C.Y., Huang C.C., Hwang C.C., Hsu T.W., Ku Y.L., Wang W.K., Hung C.Y., Chiang T.Y., 2012. Isolation and characterization of microsatellite loci from Pinus massoniana (Pinaceae). Botanical studies 53: 191-196.
7. Lê Đình Phương, 2013. Nghiên cứu một số đặc điểm sinh vật học và kỹ thuật gieo ươm loài Giổi ăn quả(Michelia tonkinensis A.Chev.) tại Vườn quốc gia Bến En, tỉnh Thanh Hóa. Luận văn thạc sỹ khoa học Lâm nghiệp, Trường Đại học Lâm nghiệp.
8. Li C.H., Hidayat M.Y., Fong Y.K., Hong Y., 2010. Isolation and characterization of 18 polymorphic microsatellite loci for Pterocarpus indicus (Leguminosae). Molecular Ecology Resources 10:1098-1105.
9. Mellick R., Porter C., Rossetto M., 2009. Isolation and characterisation of polymorphic microsatellite loci from Podocarpus elatus (Podocarpaceae). Molecular Ecology Resources 9(6): 1460 -1466.
10. Miao Y., Lang X., Li S., Su J., Wang Y., 2012. Characterization of 15 polymorphic microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae), a conifer of medicinal importance. Int. J. Mol. Sci. 13: 11165-11172.
11. Nguyễn Hoàng Nghĩa, Nguyễn Đức Thành, Lê Thị Bích Thủy, 2010. Phân tích đa dạng di truyền loài Giổi xương (Michelia baillonii (Pierre) Fin.et Gagnep) bằng chỉ thị phân tử RAPD và cp SSR. Tạp chí Khoa học Lâm nghiệp.
12. Peakall R., Smouse P.E., 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.
13. Rohlf F.J., 1992. NTSYS-PC: Numerical taxonomy and multivariate analysis system version 2.0. State University of New York (Stony Brook, New York).
14. Sun Y., Liu Y.F., Wang J., Guo Y., Huang H.W., 2010. Ten polymorphic microsatellite markers in Michelia maudiae (Magnoliaceae). American Journal of Botany: e157-e158.
15. Sun Y., Wen X.Y., Huang H.W., 2011. Genetic diversity and differentiation of Michelia maudiae(Magnoliaceae) revealed by nuclear and chloroplast microsatellite markers. Genetica 139: 1439-1447. DOI: 10.1007/s10709-012-9642-0.
16. Triệu Văn Hùng, 2007. Lâm sản ngoài gỗ Việt Nam. Dự án hỗ trợ chuyên ngành lâm sản ngoài gỗ tại Việt Nam- Pha. II.
17. Vendramin G.G., Lelli L., Rossi P., Morgante M., 1996. A set of primers for the amplification of 20 chloplast microsatellites in Pinaceae. Mol. Ecol. 5: 595-598.
18. Zhang X., Shen S., Wu F., Wang Y., 2017. Inferring genetic variation and demographic history of Michelia yunnanensis Franch. (Magnoliaceae) from chloroplast DNA sequences and microsatellite markers. Frontiers in Plant Science 8: 583. doi: 10.3389/fpls.2017.00583.
19. Zhang X., Ye W.H., Cao H.L., Wang Z.F., Shen H., Lian J.Y., 2009. Isolation and characterization of microsatellites in Chinese white olive (Canarium album) and cross-species amplification in Canarium pimela.Conserv Genet. DOI 10.1007/s10592-009-9827-y.
20. Zhao X.F., Ma Y.P., Sun W.B., Wen X.Y., Milne R., 2012. High genetic diversity and low differentiation of Michelia coriacea (Magnoliaceae), a critically endangered endemic in Southeast Yunnan, China. Int. J. Mol. Sci. 13: 4396-4411. DOI: 10.3390/ijms13044396.
21. Zhao X.F., Sun W.B., Yang J.B., Meng J., 2009. Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China. Conserv. Genet. 10:1583-1585. DOI 10.1007/s10592-008-9799-3.
