Regeneration of acacia hybrid through callus and somatic embryogenesis for gene transfer
Keywords:
Acacia hybrid, , callus, somatic embryogenesis,, regeneratioAbstract
Research on regenerating acacia hybrid through callus and somatic
embryogenesis is important, serving the gene transfer into this plant.
Results of research on creating callus tissue from the young shoot segments
and leaf pieces of 3 clones of hybrid Acacia BV10, BV16 and BV33
resulted ratio of callus induction from 88.3% to 93.6% in MS medium
supplemented with 1 mg/l 2.4-D and 0.5 mg/l BAP. In MS medium
supplemented with 1 mg/l TDZ and 0.25 mg/l IAA, the percentage of callus
that formed somatic embryos ranges from 52.6% to 59.3%; the percentage
of somatic embryos that developed shoots reached 56.7% to 58.4% after 9
weeks of culture. MS medium supplemented with 1.5 mg/l BAP and 0.5
mg/l NAA is suitable for multiplication with an average of 6.5 to 7.2
shoots/cluster. Appropriate rooting medium is 1/2MS supplemented with
1.5 mg/l IBA with rooting rates from 88.33% to 90.83%. Acacia hybrid
regeneration system through callus and somatic embryogenesis can be
applied for gene transfer to create new varieties with desired traits
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