In vitro propagation of Acacia auriculiformis A. Cunn. ex Benth by tissue culture technique
Keywords:
Acacia auriculiformis, micro -propagation, tissue culture, axillary shoot, adventitious shoot, multiplication rate and rootingAbstract
Micropropagation is an useful technique for mass propagation in clonal forestry. Study on tissue culture propagation to optimize protocol and supply genetically improved varieties for plantations of some selected clones of A. auriculiformis, such as Clt18, Clt7, Clt26, and Clt57 have been conducted. The process was started with explant sterilization using HgCl2 at
0.1% and soaked segments of axillary shoots in 5 minutes. The result achieved 31.9% of shoot proliferation and 40.1% of contamination. The medium MS* + 1.0mg/l BAP + 0.50mg/l NAA was sucessfully used for inducing the adventitious shoots with maximum 6 shoots per clump, which equals to average multiplication rate of 2.1 and adventitious shoot percentage of 48.3%. The best rooting responses were observed in the
medium 1/2MS* supplemented with 2.0mg/l IBA and the rooting rate reached to 95.3%. Other option for rooting was in vivo root by using the commercial product named as TTG containing 1.0% IBA for the standard microshoots. The rooted plantlets were acclimatized in 6 - 10 days before transferring to nursery and obtained successfully survival rate up to 85.9%.
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