Genetic diversity assessment and genetic resources indentification of (Scaphium macropodum (Miq) using moleculer markers
Keywords:
Genetic diversity,, gene resources, ITS (Internal Transcribed Spacer) marker, Scaphium macropodumAbstract
Scaphium macropodum is a multi-purpose species that grows fast and is
very valuable in Vietnam. However, the exploitation of this species by
cutting branches is popular at present, triggering this species for being in
danger of being threatened. Therefore, in this study, we have used the ITS
marker to sequencing and have identified 25 sequence segments of 25 leaf
samples of S. macropodum collected from Quang Nam, Quang Ngai and
Thua Thien Hue provinces that have similarities in the range from 94.01%
to 94.46% with reference sample AY083663.1 Scaphium lychnophorum. At
the same time, research results have also determined that the genetic
similarity of the nucleotide sequence of the samples is very high (ranging
from 97.96 - 99.85%). Based on the phylogenetic tree, 25 S. macropodum
samples in the study and the reference sample were divided into two main
groups. The results of this study are very important for the proper
exploitation and the conservation of the genetic resources of this important
indigenous plant species as well
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