EVALUATION OF GENETIC DIVERSITY OF Calophyllum inophyllum L. IN SOME SOUTHERN PROVINCES
Keywords:
Calophyllum inophyllum L.,, DNA, genetic diversity, ISSR markersAbstract
The aim of this study is evaluating the genetic diversity of Calophyllum inophyllum L. of some Southern
provinces in order to build up the genetic conservation and development strategies for this mutli- purposes
species. Five ISSR markers were used to amplify the genomic DNA of 80 samples of C. inophyllum L. that
were collected from Ben Tre, Tra Vinh, Soc Trang, Bac Lieu and Ca Mau. The results showed a total of 43
DNA segments were obtained, in which 27 polymorphic segments accounting for an average of 63.72%. The
average genetic diversity indexes of C. inophyllum L. samples obtained were h = 0.2167, I = 0.329 and PPB =
63.72%, respectively. In which, C. inophyllum L. populations in Ben Tre and Tra Vinh provinces have higher
values than studied populations. There was genetic difference between populations of C. inophyllum L. in 5
provinces in the South with GST = 0.2490 (24.90%) and Nm = 1.5083. The genetic distance between the 5
studied populations of C. inophyllum L. ranges from 0.069 to 0.190 and the degree of similarity ranges from
0.827 (82.70%) to 0.934 (93.40%). Ben Tre, Tra Vinh and Soc Trang populations had higher genetic diversity
than that in Bac Lieu and Ca Mau provinces. Therefore, research and development should be focused on
research and development genetic resources of these populations the species.
References
1. Chopra R.N., Nayar S.L., Chopra I.C., 1956. Glossary of Indian Medicinal Plants, CSIR, New Delhi, pp.46.
2. Deng, S., Fu, C., Chang, K., Yang, C., & Huang, C., 2017. Population genetic variations of Calophyllum inophyllum in Taiwan and on nearby islands. Taiwan Journal of Forest Science, 32(2), 145 - 157.
3. Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19 (1), p. 11 - 15.
4. Isshiki S., Iwata N., Khan M.M.R., 2008. ISSR variations in eggplant ( Solanum melongena L.) and related Solanum species. Scientia Horticulture 117: 186 - 190.
5. Kumar S, Stecher G, Li M, Knyaz C, Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. Jun 1; 35(6):1547 - 1549
6. Palanikumaran, B., Parthiban, K. T., Renganayaki, P. R., & Fernandaz, C. C., 2019. Genetic Diversity Studies on Calophyllum inophyllum (Undi) Progenies Through Inter Simple Sequence Repeat Markers. Agroforestry for Climate Resilience and Rural Livelihood, 399.
7. Peakall, R. DNA P.E. Smouse, 2006. Genalex 6: genetic analysis in Excel. Population genetic software for teaching DNA research. Molecular Ecology Notes, 6(1): p. 288 - 295.
8. Prabakaran K. and John Britto S., 2012. Biology, agroforestry and medicinal value of Calophyllum inophyllum L. Clusiacea): a review. International Journal of Natural Products Research. 1 (2). P. 24 - 33.
9. Rajesh Gunaga, Vasudeva R., 2011. Enhancement of seed germination through proper pre-sowing treatment inCalophyllum inophyllum, an important forest resource of the western ghats. Karnataka Journal of Agricultural Sciences, 24(3), p.413 - 414.
10. Sanjaykumar Dalvi, Swati Sonawane, and Raghunath Pokharkar, 2012. Preparation of Biodiesel of Undi seed with In-situ Transesterification. Leonardo Electroic Journal of Practices and Technologies, Issue 20, JanuaryJune 2012, p.175 - 182.
11. Shinde PP, Rane AD, Bhave SG, Gunaga RP and Narkhede SS, 2012. Variability and Genotype Selection in Calophyllum inophyllum for Quanlity Fruit Yield in the Central West Coast of India. Journal of Tree Science, Volume 31, No.1&2, p:8 - 14.
12. Spino C, Dodier M, Sotheeswaran S., 1998. Anti-HIV coumarins from Calophyllum seed oil. Bio-org. Med. Che,. Lett., 8, p.3475 - 3478.
13. Yeh FC, Yang RC, Boyle T., 1999. POPGENE Vers. 1.31: Microsoft Windows-based Freeware for Population Genetic Analysis. Quick user’s guide. City Canada; Univ. of Alberta
