In vitro propagation techniques of new eucalyptus hybrid clones (Eucalyptus urophylla  Eucalyptus pellita) UP223, UP171 and UP164

Abstract

Study on propagating of new eucalyptus hybrid clones (Eucalyptus
urophylla  Eucalyptus pellita) UP223, UP171 and UP164 by in vitro
propagation techniques was investigated. The results have the potential to
contribute to completing the process of selecting and creating new
Eucalyptus hybrids. The results of the research on sterilization phase to
produce in vitro clean samples and rapidly multiply shoots of three
eucalyptus hybrid clones showed that: sterilization of samples with 0.05%
HgCl2 in 6 minutes provided the highest effective rate of shoots with all eucalyptus lines. The effective budding of UP223, UP171 and UP164 were
32.6%; 34.4% and 31.2% respectively; while sample sterilization with
2.5% javen in 8 minutes archieved 20.4%; 21.1% and 19.5% respectively.
Effective shoots were regenerated in modified Murashige and Skoog
medium (MS*) supplemented in MS* + 0.5 mg/l BAP + 0.5 mg/l kinetin
(shoot mutilplier of UP223 was 2.84 while average shoot hight was
2.87cm; these values for UP171 line were 2.93 and 2.98 cm, respectively.
The shoot mutilplier and average shoot hight of UP164 line were 2.77 and
2.75 cm, respectively). The suitable rooting medium for UP223 line was
1/2 MS* + 30 g/l sugar + 5.5 g/l Agar +1.5 mg/l IBA + 0.75 mg/l ABT +
100 mg/l AC (actived charcoal) to get the rooting rate of 88.9% and the
number of roots/buds was 3.88. Medium for UP171 and UP164 lines were
1/2 MS* + 30 g/l sugar + 5.5 g/l Agar + 1.5 mg/l IBA + 0.5 mg/l ABT +
100 mg/l AC to have 90% rooting rate and number of roots/buds was 3.91.
The nursering time was 15 days with the survival rate from 85.6 - 88.9%,
the height growth reached 5.03 - 5.22 cm.