GENETIC DIVERSITY AND GENETIC STRUCTURE Platanus kerrii Gagnep IN SOME NORTHERN PROVINCES USING ISSR MARKERS
DOI:
https://doi.org/10.70169/VJFS.1177Keywords:
Endemic, genetic diversity, genetic structure, ISSR, Platanus kerrii GagnepAbstract
Platanus kerrii Gagnep is an endemic tree species in Vietnam with scientific, conservation, medicinal values and also great economic potential. Until now, studies have only focused on distribution, forestry characteristics and planting techniques for the species. Therefore, assessing the genetic diversity of existing gene sources is necessary to guide the future conservation and development of gene sources of this tree species. This study analysed 30 samples collected in 3 origins of Hoa Binh, Thai Nguyen, and Bac Kan using 10 ISSR markers to assess the level of genetic diversity and population structure of existing gene sources. The results showed that the genetic diversity of the species was at a medium to high level (h = 0.273) with relatively significant genetic differences between origins (GST = 0.2232), and the frequency of gene exchange between origins was not large (Nm = 1.7400). Population structure showed genetic differences between the three origins and was divided into three distinct groups, with a high proportion of concentrated samples in each group. The results of this study contribute to the future conservation and development of the genetic resources of this plant species.
References
1. Allendorf, E.F., Funk, C. W, Aitken, S. N., Byrne, M., Luikart, G., 2022. Conservation and the Genomics of Populations. Oxford University Press, London, UK.
2. Bộ Khoa học Công nghệ, 2007. Sách Đỏ Việt Nam, phần Thực vật. NXB Khoa học tự nhiên và Kỹ thuật, Hà Nội, 302-303.
3. Earl, D.A., Vonholdt, B.M., 2012. Structure Harvester: A Website and Program for Visualizing Structure Output and Implementing the Evanno Method. Conserv. Genet. Resour. 4:359 - 361
4. Ellstrand, N.C., 1992. Gene flow among seed plant populations. New Forest 6, 241–256.
5. Galván-Hernández, D. M., Lozada-García, J. A., Flores-Estévez, N., Galindo-González, J., & Vázquez-Torres, S. M., 2015. Variation and genetic structure in Platanus mexicana (Platanaceae) along riparian altitudinal gradient. International Journal of Molecular Sciences, 16(1), 2066-2077. DOI: 10.3390/ijms16012066
6. Huang, W. J., Ning, G. G., Liu, G. F., & Bao, M. Z., 2009. Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. Biologia Plantarum, 53, 159 - 163.
7. Jordano, P., 2010. Pollen, seeds and genes: The movement ecology of plants. Heredity, 105, 329 - 330
8. Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547 - 1549. DOI:10.1093/molbev/msy096
9. Lee, J. Y., Han, M. S., & Shin, C. S., 2012. Variant identification in Platanus occidentalis L. using SNP and ISSR markers. Korean Journal of Plant Resources, 25(3), 308-316.
10. Muhammad, I. and Muhammad, I., 2014. Molecular markers in plants for analysis of genetic diversity: A review. European Academic Research, 2(1), 1513–1540.
11. Peakall, R. O. D., & Smouse, P. E., 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular ecology notes, 6(1), 288 - 295. DOI: 10.1111/j.1471-8286.2005.01155.x
12. White, T. L., 2007. Forest Genetics. CAB International.
13. Yeh, F. C., Yang, R. C., Boyle, T. B. J., Ye, Z. H., & Mao, J. X., 1997. POPGENE, the user-friendly shareware for population genetic analysis, version 1.31. Molecular Biology and Biotechnology Centre, University of Alberta, Alberta.
