Research on in vitro propagation of Anthurium crystallinum

Abstract

Anthurium crystallinum is a flowering plant belonging to the family Araceae, this is a plant with high economic value. Currently, when the demand for this plant is increasing while the ability to multiply shoots in the wild is quite low and takes time, the application of in vitro propagation process in propagating this plant is the best solution to meet the material source in the future. The process of propagating A. crystallinum plants using the tissue culture method was successfully carried out in this study. Natural A. crystallinum shoots were sterilized with Javel 2% for 5 minutes and HgCl₂ 0,1% for 6 minutes to give the highest clean sample rate and shoots rate (87.78% and 79.97%). The suitable regeneration and multiplication medium for rapid multiplication of A. crystallinum is MS medium supplemented with 2.0 mg/L BAP and 0.5 mg/L NAA for an absorption coefficient of 4.85 shoots/explant, with an average height of 2.61 cm. The medium ½ MS supplemented with 0.5 mg/L NAA and 1.0 mg/L IBA is suitable for roots exudation. Ingredients containing 50% pine bark : 40% stone : 10% organic fertilizer is the best substrate for seedlings to adapt to natural living conditions, after 30 days survival rate reaches 100%.