Research on propagation Q15.38, Q15.013, Q16.427 clones of Melaleuca quinquenervia by technique in vitro

Abstract

Q15.38, Q15.013, Q16.427 clones of Melaleuca quinquenervia (Cav.) S.T.
Blake have high content and quality of essential oils, recognized as
technically advanced varieties by the Ministry of Agriculture and Rural
Development. Therefore, identification and optimization of the in vitro
propagation method for the above clones by in vitro culture method are
necessary and have practical significance in increasing the biodiversity and
safety in the industrial afforestation, as well as to quickly put good varieties
into production and provide large quantities of stable see dlings to forest
growers. The results of in vitro propagation of these Melaleuca clone lines
showed that cleaned samples by disinfecting with Javen 5% for 10 minutes
are shooting with 44.44 - 64.4%. The medium to create shoot cluster is
MS* + 0. 7 mg/l BAP + 0.2 mg/l Kinetin +0.1 mg/l NAA, with 100% of created shoot cluster and 3.5 - 3.6 shoots per cluster. Stimulating shoot
growth on medium of 0.3 - 0.4 mg/l Kinetin + 0.7 mg/l BAP + 0.1 mg/l
NAA is resulted with effective shoot rate of over 84.44%, coefficient shoot
multiplication of over 3.7 times, shoot height of over 2.73 cm after 3 weeks
of tissue culture. The shoot pots in the rapid multiplication period placed
under 1,000 lux light intensity for 1 culture cycle are most suitable. The
rooting medium of these Melaleuca clone lines is MS* + 1.5 mg/l NAA
(100% rooting shoots, 2.4 - 3.8 roots/shoot, 1.7 - 2.2 cm root length after 3
culture weeks). The substrate of 30% hunk rice husk and 70% of soil from
layer B is suitable for the crowning of these Melaleuca clones, with a
survival rate of over 95%, the tree height of 21.67 - 25.49 cm, the plants
with good quality after 3 months of planting in the nursery.