Study on propagation of new triploid acacia (X101, X102) by tissue culture method

Abstract

Polyploid is a natural phenomenon. Polyploid in animals is rather rare.
However, it is quite common in plants, occurring in more than 70% of
flowering plants. In forestry, the selection and use of polyploid varieties is
a new direction and it is expected to have new varieties with large
differences in genotypes and phenotypes, because forest trees are often
heterozygous. Research on tissue culture method for some promising
triploid acacia lines (X101, X102) will contribute to the completion of the
triploid breeding process, as well as a basis for creating a strateg y to
improve the variety polyploid for tropical Acacia species in Vietnam.
Tissue culture method results for a number of triploid colloidal lines
showed that the use of excess shoots or axillary buds sterilized with 0 .05%
HgCl2 for 5 minutes has the highest efficiency: the survival rate (80%); the adventitious shoot rate (28.4%). Beside, the use of 3% javel solution for 20
minutes also gave a quite good result: the survival rate (70.6%); the
adventitious shoot rate (18.2%). To protect the environment and human
health, javel solution is recommended to use in disinfection. Effective
clusters of shoots were further cultured in Murashige and Skoog modified
medium (MS) with supplementation of individual or synthetic growth. The
highest number of shoots/cluster was achieved in MS * + 1.0 mg/l BAP +
0.25 mg/l GA3 (5.0 - 5.5 shoots/cluster) and the rate of effective shoots
(48.6 - 51.9%). Effective shoots were up to the standard for rooting in
1/2MS * + 2 mg/l IBA, the rooting rate was 78.4 - 80.1%.