Introdution of the EcHB1 gene into E. urophylla × E. pellita hybrid via Agrobacterium tumefaciens
Keywords:
gen EcHB1, transgenic, wood fiber length, EcHB1 geneAbstract
The gene EcHB1, is isolated from E. grandis, encoding for increasing the wood fiber length was transferred into superior clones of E. urophylla × E. pellita (UP hybrid) via Agrobacterium tumefaciens. Use A. tumefaciens concentration of OD600 = 0.5 to transform the EcHB1 gene in the suspension solution with 100 µM Acetosyringone into UP hybrid for 10 mins. After 72 hours of co -culture, the transgenic stem and leaf segments were regenerated on MS* selective medium supplemented with 0.5 mg/l BAP, 0.2 mg/l NAA, 400 mg/l Cefotaxim and 150 mg/l Kanamycin. After three selections on the regenerative medium, transgenic UP hybrid shoots were transplanted on selective rooting medium supplemented with 50 g/l Kanamycin. Results of the analysis of nursery plants by PCR method confirmed the presence of the EcHB1 gene in the UP hybrid tree
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