EVALUATION OF THE EFFECTS OF CALLUS-MEDIATED REGENERATION ON GROWTH AND MORPHOLOGY IN SELECTED HYBRID ACACIA CLONES

Abstract

This study evaluated the differences in morphology and growth abilities of Acacia hybrid explants that regenerated via callus formation with those from in vitro cultured in the same culture and nursery conditions. The results showed that fast-growing A. hybrid clones 6.08 - 8.72 shoots/clump, plant height ranged from 3.79 - 4.16cm, thick shoots, internodes, open and green leaves. When rooting, A. hybrid clones rooted rates of over 85%, with well-developed roots, white color, and light yellow root tips. After 3 months in the nursery, A. hybrid clones have rates of over 90%, sturdy, healthy plants, no truncated tops, green leaves and no signs of pests or diseases. The use of the initial source of materials as regenerated callus and in vitro cultured in the stages of rapid shoot multiplication, rooting and tree formation in the nursery up to 3 months old has not had a clear effect on the growth and morphology of A. hybrid clones. The level of variation in morphology and growth between A. hybrid clones at these stages is not different, however, these are only initial assessments, so it is necessary to conduct experimental planting for more accurate assessments and comparisons.