SURVEILLANCE OF ANTAGONISTIC ENDOPHYTES AND ANTIFUNGAL COMPOUNDS OF ACACIA MANGIUM CLONES TRIALED IN THUA THIEN HUE PROVINCE
Keywords:
Acacia mangium, Antifungal compounds,, Ceratocystis sp.,, Corticium salmonicolor, endophytes,, Diease resistanceAbstract
Acacia mangium is an increasingly important plantation species in Vietnam providing industrial
wood for domestic and export markets. Pink disease caused by Corticium salmonicolor and
crown wilt caused by Ceratocystis sp.are serious threats to plantation productivity in Thua Thien
Hue. Surveillance of antagonistic endophytes and antifungal compounds in A. mangium has
potential for the initial screening for resistance to these pathogens. Therefore, a clonal A.
mangium trial planted in Thua Thien Hue province in 2009 was used to identify potential resistant
lines. Small twig samples (1 cm in diameter and about 10 cm in length) and phyllode samples
(about 1 kg) from one tree representative of each of the 35 clones in the field trial were collected
for isolating fungal and bacterial endophytes and for extracting chemical compounds using
methanol and ethylene chloride as solvents. Eight bacterial strains and 13 fungal strains were
isolated, in which 13 fungal strains and 2 bacterial strains showed antifungal activities to
Ceratocystis sp. and only 8 strains (7 fungal strains and 1 bacterial strain) expressed antifungal
activities to Corticium salmonicolor in vitro. Using the phyllodes extract residue, 26 A. mangium
clones showed high (40 – 60% suppression of diameter growth) or very high (>60%) antifungal
activities to Ceratocystis sp.; and 18 clones showed high or very high antifungal activities to
Corticium salmonicolor. Combining the endophytes and residue results, 28 of the A. mangium
clones (AMD01, AMD02, AMD04, AMD05, AMD06, AMD07, AMD08, AMD09, AMD10,
AMD11, AMD12, AMD13, AMD14, AMD15, AMD16, AMD18, AMD19, AMD20, AMD22,
AMD24, AMD25, AMD27, AMD29, AMD31, AMD32, AMD33, AMD34 and AM35) showed
promising antifungal activity to the 2 pathogens tested. Work is in progress to determine the
resistance levels of the acacia clones to the two pathogens in the field
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