Study on synnenmata production of snowflake Dongchunghacho (Isaria tenuipes) on artificial substrates
Keywords:
Snowflake Dongchunghacho, Isaria tenuipes,, synnenmata, silkwormAbstract
Isaria tenuipes is a fungus called snowflake Dongchunghacho due to its appearance that was collected from the natural forest of Hoang Lien National Park. Two kinds of artificial substrates including chemical broth medium and silkworm larva/pupa were invertigated for synnenmata production. The chemical broth medium containing 40 g of glucose + 10 g of peptone + 0.5 g of KH2PO4 + 0.5 g of K2HPO4: 3H2O, + 0.5 g of MgSO4.7H2O + 10 g of yeast extract in 1 liter H2O and medium 30 g glucose + 3 g peptone + 0.5 g KH2PO4 + 0.5 g (NH4)2SO4 + 0.5 g MgSO4.7H2O + 1 g yeast extract + 0.5 mg B1 in 1 liter H2O. When the synenmata production was cultured on silkworm pupa/larva, it was best to fungal infection from 5th instar
silkworrm larva. Synnemata production of I. tenuipes was excellent in 5th instar silkworrm larva with an incidence rate of 76.33%. Synnemata living weight obtained 0.895 g/1 pupae. The synnenmata were milky-white in color, and were similar in shape and color to wild synnemata collected in Hoang Lien Natural Park.
References
1. Phạm Quang Thu. Nguyễn Mạnh Hà. Lê Thị Xuân, 2011. Phân bố nấm Đông trùng hạ thảo Isaria tenuipes(Peck.) SamSon ở Việt Nam. Tạp chí Nông nghiệp & Phát triển nông thôn. Bộ Nông nghiệp & Phát triển nông thôn số 9. trang 93 - 97.
2. Yokoyama, E., Yamagishi, K., & Hara, A., 2003. Structures of the mating-type loci of Cordyceps takaomontana. Applied and environmental microbiology, 69(8), 5019-5022.
3. Kang, P. D., Sung, G. B., Kim, K. Y., Kim, M. J., Hong, I. P., & Ha, N. G., 2010. Breeding of a silkworm variety for synnemata production of Isaria tenuipes. Mycobiology, 38(3), 180-183.
4. Oh.. G. S.. K. H. Hong. H. Oh. H. O. Pae. I. K. Kim. N. Y. Kim. T. O. Kwon. M. K. Shin. and H. T. Chung., 2001. 4-Acetyl-12.13-epxyl-9-trichothecene-3.15-diol isolated from the fruiting bodies of Isaria japonica Yasudainduses apoptosis of human leukemia cells (HL-60). Boil. Pharm. Buil. 24: 785- 789.
