Isolation and Evaluation of Endophytes from Acacia mangium antagonising to Ceratocystis manginecans
Keywords:
Acacia mangium, Ceratocystis manginecans, endophytes, wilt diseaseAbstract
Acacia species are planted for sawnwood, chip and pulp. In Vietnam, Acacia hybird, A. mangium and A. auriculiformis have been planted in large scale under areas of about 1.3 million hectares in 2015. However, the wilt disease caused by Ceratocystis manginecans has been spread and become a serious threat to these plantations. The result of a study undertaken in order to develop the bioproduct to control the disease showed that 14 endophytic bacterial strains and 12 fungal endophyte strains were isolated from A. mangium planted in Thai Nguyen province. These strains were used to test antifungal activity by using the dual culture method. The antifungal activity of the bacterial endophytes against C. manginecans differed between strains and showed that two endophytic bacterial strains (K1 and K7), and two endophytic fungal strains (N28 and N31) showed very strong antagonism to C. manginecans. Endophytes was identified by molecular biology technique. The endophytic bacterial strain K1 was indicated as Bacillus cereus, K7 was indicated as Bacillus tequilensis. The fungal endophyte strain N28 was indicated as Diaporthe tectonigena and N31 was indicated as Arcopilus sp. However, the bacterial B. cereus has been identified as a cause of food poisoning, causing diarrhea. It is recommended that three endophytes (Bacillus tequilensis, Diaporthe tectonigena and Arcopilus sp. (N31)) be considered for managing Ceratocystis wilt disease.
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