Study on regeneration system by somatic embryogenesis of sellected hybrids of Eucalyptus urophylla tree serving for transgenis works
Keywords:
Eucalyptus uropylla hybrid, somatic embryogenesis, tissue cultureAbstract
Regeneration from somatic embryogenesis for selected clone of Eucalyptus urophylla hybrid have been successfully studied. Materials for regeneration study were obtained from leaves and stem segments of in - vitro shoot after subculture of 18 - 22 days. The study results showed that percentages of
callus formation on MS medium supplement with 2.4D 4mg/l and BAP 3mg/l are 87.9% và 90.5 % for stem segments and leaves, respectively. Callus clusters were transfered to embryonic induction medium with BAP 4mg/l and NAA 0.5 mg/. Embryonic cluster continue to be transfered to shoot induction medium containing BAP 1mg/l và NAA 0.5mg/l for six
weeks. The medium has shoot induction abilities are 53.3% and 62.9% from stem segments and leaves (respectively). Shoots were then transfered to root induction medium (1/2MS + IBA 2mg/l and NAA 1mg/l) to form complete plant and this medium has highest rooting rate of 78.3%
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