RAPD ANALYSIS OF THE GENETIC DIVERSITY OF NATURAL POPULATION OF Rhomboda lanceolata (Lindl.) Ormd FROM LAM DONG PROVINCE

Authors

  • Nguyen Thuy Ha University of Da Lat
  • Nong Van Tiep University of Da Lat
  • Le Ngoc Trieu Centre for Applications Nuclear Techniques in Industry
  • Nong Van Duy Tay Nguyen Institute of Biology
  • Tran Van Tien Lam Dong Silvicultural Experimentation Research centre Vietnam Academy of Forest Sciences

Keywords:

Rhomboda lanceolata (Lindl.) Ormd, Genetic diversity, RAPD

Abstract

Three generations of the population of Rhomboda lanceolata (Lindl.) Ormd were collected in
Lang Bian mountain, Lam Dong province, Vietnam for analysis of genetic diversity. In this
research, random amplified pol morphic DNA (RADP) makers were employed to investigate
the genetic variability in 30 individuals of that population, which corresponded to above three
generation. With 11 primers, 28 highly reproducible and clear RAPD bands were obtained.
The percentage of polymorphic loci at species level in the research was significantly quite
low (Pt = 76.92%); This percentage at population level was also low and ranged from 59.23 % to
64.61%. However, the first generation has a higher % P (P1 = 64.61%) than its generative
congener. Significant heterozygosity at species level was quite low (HEt = 0.2692),but, at
populations level, there was markly differences between the generations. The first generation has
higher values of diversity (HE1 =0.2960, in generation 1; HE2 = 0.190, in generation 2; HE3 =
0.1893, in generation 3). Genetic variation within population was significantly quite low, with GST
= 0.2125. Genetic distance between generations of that population were remarkably differentiated,
for example: genetic distances between generations 1 and 2 was D12 = 0.49; genetic distances
between generations 1 and 3 was D13 = 0.51 and genetic distances between populations 2 and
3 was D23 = 0.48. Result of UPGMA cluster analysis were recorded 4 group (including,
generation 1, 2, 3 and hybridized generations).

References

Anatonovis J., 1984. Genetic variation within population. In Dirzor R., Sarukan J. (eds)

perspectives on plant population biology. Sinauer, Sunderland, 229-241.

Ang L., Luo Y. B., Xiong Z. T. and Song G. E., 2002. A Preliminary study on conservation

genetics of three endangered Orchid species. Acta Botanica Sinica, 44(2): 250-252.

Averyanov L. V. & Averyanova A. L., 2003. Updated checklist of the orchids of Vietnam.

Vietnam National University Publishing House, Hanoi, Vietnam.

Beardmore J.A., 1983. Extinction, survival and genetic variation. In: Schoenwald-Cox C.M.,

Chamber S.M., Macbryde B., Thomas L. (eds). Genetics and Conservation. BenjaminCummings, Menlo Park, 125-151.

Carmen M., Teresa P., Margarita C. M. and Esteban H. B., 2008. Genetic Diversity and

Structure of the Endangered Betula pendula subsp. fontqueri Populations in the South

of Spain. Silva Fennica 42(4): 487–498.

Ceska J. P., Afollter J. M. and Hamrich J. C., 1997. Developing a sampling strategy for

Raptisia arachiifera based on allozyme diversity. Conservation Biology, 11: 1133-

Dharmar K. and John A. D. B., 2011. RAPD analysis of genetic variability in wild

populations of Withania somnifera (L.) Dunal. International Journal of Biological

Technology, 21-25.

Hogbin P. M. and Peakall R., 1999. Evalution of the contribution of the genetic research to

the management of the endangered plant Zieria prostrate. Conservation Biology, 13:

-522.

Jennifer M. C. and Hamrick J. L., 2004. Genetic Diversity in Harverted and Protected

Populations of Wild American Ginseng, Panax Quinquefolius L. (ARALIACEAE).

American Journal of Botany, 91(4): 540–548. 2004.

IPGRI and Cornell University, 2003. Measures of genetic diversity.

Kurt W., Hilde N., Kirsten W. and Kahl G., 2005. DNA Fingerprinting in plants principles,

methods, and applications (second Edition). Cpc press Taylor & Fancies group.

Lynch M. and Milligan B. G., 1994. Analysis of population genetic structure with RAPD

markers. Molecular Ecology, 3 (2): 91–99.

Milligan B. G., Leebens-M. J. and Strand A. E., 1994. Conservation genetics: Beyond the

maintenance of marker diversity. Molecular Ecology, 12: 844–855.

Nei. M., 1972. Genetic distance between populations. American Naturalist 106: 283-292.

Nei M., 1978. Estimation of average heterozygosity and genetic distance from a small number

individual. Genetics 9: 583-590.

Nei M., and Tajima F., 1981. DNA polymorphism detectable by restriction endonucleases.

Genetics 97: 145-163.

Nei M., and Tajima F., 1983. Maximum likelihood estimation of the number of

nucleotide substitutions for restriction sites data. Genetics 105: 207-216.

Nei M. and Jin L., 1989. Variances of the average numbers of nucleotide substitutions

within and between populations. Molecular Biology Evolution 6: 240-300.

Nei M. and Miller J. C., 1990. A Simple Method for Estimating Average Number of

Nucleotide Substitutions Within and Between Populations From Restriction Data.

Genetics Society of America. 125: 873-879.

Pvingila D., Verbylaitë R., Baliuckas V., Pliûra A. and Kuusienë S., 2005. Genetic diversity

(RAPD) in natural Lithuanian populations of common ash (Fraxinus excelsior L.).

Biologija, 3: 46–53.

Sergei V. Bahtiyour Y., Irina S., David W. Varda Z. and Samuel M.et al., 2001. Tests for

adaptive RAPD variation in population genetic structure of wild barley, Hordeum

spontaneum Koch. Biological Journal of the Linnean Society, 74: 289–303.

Steele P. R. and Pires J. C., 2011. Biodiversity assessment: State of the art techniques in

phylogenomics and species indentification. American Journal of Botany, 98:415-415.

Robichaux R. H., Friar E. A., Mount D. W. 1997. Molecular genetics consequences of a

population bottleneck associated with reintroduction of the Mauna Kea Silverwoold

(Argyroxiphium sanwicense spp. sanwicense L. [Asteraceae]. Conservation Biology,

: 1140-1146.

William J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. and Tingey S. V., 1990. DNA

polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic

Acids Reseach, 6531 - 6535.

Woff P. G. & Sinclair R. B., 1997. Highly differentiated populations of the narrow endemic

plant Maquire Primrose (Primula maguirei). Conservation Biology, 11: 375-391.

Published

14-01-2024

How to Cite

[1]
Ha, N.T. et al. 2024. RAPD ANALYSIS OF THE GENETIC DIVERSITY OF NATURAL POPULATION OF Rhomboda lanceolata (Lindl.) Ormd FROM LAM DONG PROVINCE. VIETNAM JOURNAL OF FOREST SCIENCE. 4 (Jan. 2024).

Issue

Section

Articles

Most read articles by the same author(s)

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.