RAPD ANALYSIS OF THE GENETIC DIVERSITY OF NATURAL POPULATION OF Rhomboda lanceolata (Lindl.) Ormd FROM LAM DONG PROVINCE
Keywords:
Rhomboda lanceolata (Lindl.) Ormd, Genetic diversity, RAPDAbstract
Three generations of the population of Rhomboda lanceolata (Lindl.) Ormd were collected in
Lang Bian mountain, Lam Dong province, Vietnam for analysis of genetic diversity. In this
research, random amplified pol morphic DNA (RADP) makers were employed to investigate
the genetic variability in 30 individuals of that population, which corresponded to above three
generation. With 11 primers, 28 highly reproducible and clear RAPD bands were obtained.
The percentage of polymorphic loci at species level in the research was significantly quite
low (Pt = 76.92%); This percentage at population level was also low and ranged from 59.23 % to
64.61%. However, the first generation has a higher % P (P1 = 64.61%) than its generative
congener. Significant heterozygosity at species level was quite low (HEt = 0.2692),but, at
populations level, there was markly differences between the generations. The first generation has
higher values of diversity (HE1 =0.2960, in generation 1; HE2 = 0.190, in generation 2; HE3 =
0.1893, in generation 3). Genetic variation within population was significantly quite low, with GST
= 0.2125. Genetic distance between generations of that population were remarkably differentiated,
for example: genetic distances between generations 1 and 2 was D12 = 0.49; genetic distances
between generations 1 and 3 was D13 = 0.51 and genetic distances between populations 2 and
3 was D23 = 0.48. Result of UPGMA cluster analysis were recorded 4 group (including,
generation 1, 2, 3 and hybridized generations).
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