Study on some factors influencing the rate of initiation, proliferration and maturation of embryogenic tissues in Pinus merkusii Jung et De Vrise in vitro
Keywords:
Pinus merkusii, somatic embryogenesis,, 24 - epibrassinolide,, 2,4 - dichlorophenoxyacetic acidAbstract
Pinus merkusii is a species with high economic value, on the other hand pine has the ability to grow and thrive on poor soil, bad drought. Therefore, somatic embryogenesis techniques through immature embryos culture were studied and applied to create large volumes of flow desired trees to provide production. Factors influencing initiation and proliferation of embryonal tissues of Pinus merkussi were studied. Embryogenesis ratio 25.3% to 40.0% of somatic embryos were achieved when immature zygotic embryos were cultured on LVM medium supplemented with 2,4 - D 2.0mg/l and BA 1.0mg/l. Somatic embryo formation rate has been improved up to 37.6 - 58.3% when replaced BA 1.0mg/l by 24 -epibrassinolide 1.0mg/l. Optimum medium for fast coppiced in all lines was associated with LVM combined 2,4 - D (2,0mg/l) and BA (1,0mg/l), fresh weigh increased from 2.58 to 3.89 time in the first week. The number of somatic embryos 40.0 to
58.3/1g of fresh weight of embryogenic tissue depending on lines on maturation medium LVM combined with ABA from 80 to 90µM.
References
1. Dunstan DI; Dong J - Z; Carrier DJ & Abrams S, 1998. Events following ABA treatment of spruce somatic embryos. In vitro Cel Dev. Biol - Plant. 34: 159 - 168.
2. Ellis D, 1995. Genetic transformation of smatic embryos. In. Bajaj YBS (ed) Biotechnology in Agriculture and Forestry Vol. 3. Somatic embryogenesis.
3. Harry IS, Thorpe TA, 1991. Somatic embryogenesis and plantlet regenration from mature zygotic embryos of red spruce. Bot Gaz. 152: 446 - 452.
4. Klimazewska.K, Cardou MB; Cyr DR and Sutton BCS, 2000. Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus l. In Vitro Cell. Dev. Biol. Plant 36: 279 - 286
5. Lâm Công Định, 1977. Trồng rừng thông. Nxb. Nông nghiệp Hà Nội, 223 trang
6. Lelu MA; Bastien C; Drugeault A; Gouez ML and Klimaszewska K, 1999. Somatic embryogenesis and plantlet development. I. Pinus sylvestris and Pinus pinaster on medium with and without growth regulators. Physiol. Plant. 105: 719 - 728.
7. Lelu - Walter MA, Bernier - Cardou M, Klimaszewska K, 2008. Clonal plant production from self - and cross -pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis. Plant Cell Tissue and Organ Culture 92: 31 - 45
8. Malabadi R. B., Teixeira da Silva J. A., Mulgund G. S., 2011. Induction of somatic embryogenesis in Pinus Caribaea. Tree and Forestry science and Biotechnology 5(1): 27 - 32.
9. Maruyama E, Hosoi Y, Ishii K, 2005. Propagation of Japanese red pine (Pinus densiflora Zieb. et Zucc.) via somatic embryogenesis. Propagation of Ornamental Plants 5: 199 - 204.
10. Minocha S. C. ; Minocha, R, 1995. Historical aspects of somatic embryogenesis in woody plants. In: Jain, S. M.; Gupta, P. K.; Newton, R. J., ed. Somatic embryogenesis in woody plants. Vol. 1. Dordrecht, Netherlands: Kluwer Academic Publishers: 9 - 22.
11. Tang W, Guo ZC and Ouyang F, 2001. Plant regeneration from embryogenic cultures initiated from mature Loblolly pine zygotic embryos. In Vitro Cell. Dev. Biol - Plant 37: 558 - 563.
12. Viện khoa học lâm nghiệp Việt Nam, Viện tư vấn phát triển kinh tế - xã hội nông thôn và miền núi, 2002. Kĩ thuật trồng cây làm nguyên liệu giấy, Nxb. Lao động - Xã hội, Hà Nội.
13. Park YS., Lelu MA.,Walter L. Harvengt., Trontin JF., MacEacheron I., Klimaszewska K. & Bonga JM, 2006. Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France. Plant Cell Tiss Organ Cult 86: 87 - 101.
14. Walter C, Charity J, Grace L, Hofig K, Moller R, Wagner A,2002. Gene technologies in Pinus radiata and Picea abies: tools for conifer iotechnology in the 21st century. Plant Cell Tissue Organ Cult 70: 3 - 12
