Study on breeding of black ginger (Distichochlamys citrea) by in vitro method

Abstract

Black ginger (Distichochlamys citrea) is a new endemic plant species in
Vietnam. In nature, D. citrea is narrowly distributed in small quantities.
Essential oils could be extracted from stems, roots and leaves. The chemical
active agents include: 1.8-cineole (30.7-43.7)%, β-citral (1.6-14)%, a-citral (
2.5-20.9)% and neryl acetate (4.1-11.1)%, α-pinene (1.8-4.5)%, β-pinene
(2.8-7.0)%, D-limonene (1.2-3.3)%, Geraniol (4.0-5.9)%, Neryl acetate
(4.1-11.1)%,... Chemicals in ginger is considered to be good for health and
can be used to make cosmetics, food. Effective breeding of black ginger by
in vitro technique as follow: After sterilization, shoots were regenerated on
the medium (MS + 5g/l Agar + 30g sugar + 100ml/l coconut water + 1mg/l
PVP + 2mg/l BAP + Kn 0.2 mg/l). After regeneration, shoots were
multiplied on the medium: (MS + 5g/l Agar + 30g sugar + 100ml/l coconut
water + 1mg/l BAP + 1g/l Casein + 0.2mg/Kn). Roots were formed on the
medium (MS + 30g sugar + 100ml/l coconut water + 0.5mg/l NAA).
Young plants grown in a nursery with 75% sunlight cover. They were
grown on a substrate (Coconut mulch: Husk: Hill land) with the ratio of 2:
1: 1, respectively. After 10 days, Grownmore fertilizer (30N: 10P: 10K)
was added by spraying with 0.5g/l concentration and frequency of 7
days/time. After 3 months, the seedlings grown well with 2.7 new shoots;
138 mm high; leaves are 82 mm long and 52 mm wide.