Morphological characteristics and sequence analysis of 28s - rRNA gene of fungal species associated with the rot disease of litchi fruit in Luc Ngan -Bac Giang
Keywords:
Fruit rot disease of litch, Fusarium graminearum, 28S -rRNA gene, morphological characteristics, , Litchi chinensis SonnAbstract
Litchi (Litchi chinensis Sonn.) a specialty fruit, has an appealing natural red color, high nutritional value, and pleasant flavour. However, litchi fruit can infect some pathogenic fungi. This study aimed Isolation and identification of pathogenic fungi associated with litchi fruit rot disease in Luc Ngan - Bac Giang. The fungal strain, designated as LNT1.1 was isolated from litchi fruit and identified by some morphological characteristics and analysed by 28S -rRNA sequence nucleotide. The sequencing analysis result has showed that sequence of 28S rRNA gene of the LNT1.1 strain has 606 bp and high homology to those of some representatives of the fungi genus Fusarium (92.8 -100%). Among them, it has the highest homology with that of Fusarium graminearum strain PH - 1 (Accession number XR_893061). The sequence of 28S rRNA gene fragment of LNT1.1 strain was deposited in GenBank with accession number KU521339 and was named Fusarium graminearum LNT1.1
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