FIRST RECORD OF Beauveria bassiana, A POTENTIAL BIOCONTROL AGENT FOR MANAGEMENT OF STEM BORERS

Minh Chi Nguyen; Thi Kim Anh Dang

doi:10.70169/VJFS.1043

Authors

Nguyen Minh Chi Forest Protection Research Centre https://orcid.org/0000-0002-2345-2398
Dang Thi Kim Anh Forest Protection Research Centre

DOI:

https://doi.org/10.70169/VJFS.1043

Keywords:

Beauveria bassiana, biocontrol, Endoclita, entomopathogenic fungi, insect pest, pesticide, plantation forestry, stem borer

Abstract

Acacia spp. and Eucalyptus hybrids are widely planted in Vietnam for wood products and rural development, and the total area is now about 2.75 million hectares. As the plantation area expands, insect pests have increased and they are causing serious damage to the forest estate. However, some parasitic entomopathogens have been recorded on pest species and these may have potential for future biocontrol. Entomopathogenic fungi isolated from parasitized larvae of Endoclita salvazi (isolate B2) and E. vietnamensis (isolate B3) were identified and their pathogenicity evaluated in trials. Based on the ITS, LSU and TEF1-α sequences, isolates B2 and B3 are Beauveria bassiana. These isolates had high pathogenicity, with the percentage of parasitized larvae being 74.5-76.8%. These findings provide further evidence for the effectiveness of B. bassiana isolates for the development of biopesticides.

References

1. Buchsbaum, U., Grehan, J.R., Chen, M.Y., Chi, N.M., Pham, D.L., Khai, T.Q., Jones, L.D., Ignatev, N., 2022. New species of Endoclita (C. and. R. Felder, 1874) and first record of E. salvazi from Vietnam (Insecta: Lepidoptera: Hepialidae). Vernate, 41, 267-286.

2. Chandel, R.S., Soni, S., Vashisth, S., Pathania, M., Mehta, P.K., Rana, A., Bhatnagar, A., Agrawal, V.K., 2019. The potential of entomopathogens in biological control of white grubs. International Journal of Pest Management, 65 (4), 348-362.

3. Chi, N.M., Anh, D.T.K., Long, P.D., Bao, H.Q., Van, C.V., Cong, L.T., Quang, D.N., Loi, V.V., 2022a. Entomopathogenic fungi (Cordyceps spp.) isolated from emerging insect pests associated with plantation forests in Vietnam. Science and Technology Journal of Agriculture and Rural Development, 1 (1), 41-50.

4. Chi, N.M., Bao, H.Q., Pham, D.L., Loi, V.V., Yakovlev, R.V., 2022b. The stem borer Zeuzera multistrigata Moore (Lepidoptera, Cossidae): a serious pest undermining Eucalyptus plantations in Northern Vietnam. Ecologica Montenegrina, 60, 4-12.

5. Chi, N.M., Hai, P.H., Duong, L.A., Quynh, D.N., Thu, P.Q., 2022c. Heart rot disease and screening of disease tolerance of Acacia mangium. Vietnam Journal of Forest Science, 5, 90-99.

6. Chi, N.M., Huong, V.D., Pham, D.L., Binh, L.V., Luu, N.V., Ha, K.M., Loi, V.V., Yakovlev, R.V., 2022d. Neurozerra conferta (Lepidoptera: Cossidae) damaging Melaleuca plantations in Vietnam and its biological control. Ecologica Montenegrina, 60, 13-24.

7. Chi, N.M., Nhung, N.P., Loi, V.V., Thuy, P.T.T., Dell, B., 2025. Bacillus bombysepticus, an entomopathogen in yellow‑spined bamboo locust with biocontrol potential. Neotropical Entomology, 54 (1), 1-9.

8. Chi, N.M., Pham, D.L., Nhung, N.P., Hoa, N.T.H.H., Do, T.T., Tra, T.T.L., Loi, V.V., Thuy, P.T.T., Hai, N.D., Tuan, D.X., Thu, P.Q., Dell, B., 2023. Integrated pest management of Hypsipyla robusta shoottip borer (Lepidoptera: Pyralidae) in Chukrasia tabularis (Sapindales: Meliaceae). Journal of Economic Entomology, 116 (2), 486-495.

9. Chi, N.M., Thu, P.Q., 2024. A review of Ceratocystis canker and wilt pathogens. Journal of Forestry Science and Technology, 13 (1), 22-32.

10. EFSA, 2015. Peer review of the pesticide risk assessment of the active substance Beauveria bassiana strain 147. EFSA Journal, 13 (10), 4261.

11. EFSA, 2020. Peer review of the pesticide risk assessment of the active substance Beauveria bassiana strain 203. EFSA Journal, 18 (11), e06295.

12. Enkerli, J., Widmer, F., Keller, S., 2004. Long-term field persistence of Beauveria brongniartii strains applied as biocontrol agents against European cockchafer larvae in Switzerland. Biological Control, 29 (1), 115-123.

13. Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series, vol 41. [London]: Information Retrieval Ltd., 95-98.

14. Hung, T.X., Thu, P.Q., Chi, N.M., Binh, L.V., Dell, B., 2022. Impacts and trapping of ambrosia beetles Euwallacea fornicatus and E. similis in Acacia plantations in Vietnam. Southern Forests, 84 (3), 242-252.

15. Hussein, K.A., Abdel-Rahman, M.A.A., Abdel-Mallek, A.Y., El-Maraghy, S.S., Joo, J.H., 2012. Pathogenicity of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella. Phytoparasitica, 40 (2), 117-126.

16. Kessler, P., Enkerl, J., Schweize, C., Keller, S., 2004. Survival of Beauveria brongniartii in the soil after application as a biocontrol agent against the European cockchafer Melolontha melolontha. BioControl, 49 (5), 563-581.

17. Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111-120.

18. Kumar, S., Stecher, G., Tamura, K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870-1874.

19. Mascarin, G.M., Jaronski, S.T., 2016. The production and uses of Beauveria bassiana as a microbial insecticide. World Journal of Microbiology and Biotechnology, 32 (11), 177.

20. Moro, G., Atzeni, R., Al-Subhi, A., Marche, M.G., 2025. CompàreGenome: a command-line tool for genomic diversity estimation in prokaryotes and eukaryotes. BMC Bioinformatics, 26 (1), 14.

21. Nambiar, E.K.S., Harwood, C.E., Kien, N.D., 2015. Acacia plantations in Vietnam: research and knowledge application to secure a sustainable future. Southern Forests, 77 (1), 1-10.

22. Old, K.M., See, L.S., Sharma, J.K., Yuan, Z.Q., 2000. A manual of diseases of tropical acacias in Australia, South-East Asia and India. CIFOR:

23. Pham, D.L., Chi, N.M., Grehan, J.R., Khai, T.Q., Ignatev, N., Jones, L.D., Mielke, C.G., Quang, D.N., Yakovlev, R., 2024. Notes on Endoclita C. & R. Felder (Lepidoptera: Hepialidae) feeding on Eucalyptus in Vietnam, with new records and a new species. Zootaxa, 5453 (1), 63-85.

24. Quang, D.N., Thu, P.Q., Chi, N.M., Binh, L.V., Thong, N.Q., Thu, N.H., Nguyen, V.D., Dell, B., 2021. Management of needle-eating caterpillars associated with Pinus massoniana and P. merkusii in Vietnam. Forests, 12 (11), 1610.

25. Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B., Spatafora, J.W., 2007. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology, 57, 5-59.

26. Thu, P.Q., Hung, T.X., Dinh, P.X., Binh, V.D., Chi, N.M., 2023. Insect pests and disease in Acacia plantations in North Central, Vietnam. Vietnam Journal of Forest Science, special issue, 132-140.

27. Thu, P.Q., Quang, D.N., Chi, N.M., Hung, T.X., Binh, L.V., Dell, B., 2021. New and emerging insect pest and disease threats to forest plantations in Vietnam. Forests, 12 (10), 1301.

28. To, X.P., Tran, L.H., Cao, T.C., 2021. The source of Acacia wood in Vietnam, current status and trends. Vietfores

29. Tra, T.T.L., Chi, N.M., Anh, D.T.K., Thu, P.Q., Nhung, N.P., Dell, B., 2022. Bacterial endophytes from Chukrasia tabularis can antagonize Hypsipyla robusta larvae. Phytoparasitica, 50 (3), 655-668.

30. Valero-Jiménez, C.A., Faino, L., Spring in’t Veld, D., Smit, S., Zwaan, B.J., van Kan, J.A.L., 2016. Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes. BMC Genomics, 17 (1), 986.

31. Zimmermann, G., 2007. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology, 17 (6), 553-596.