NEMATODE ISOLATES OF Caenorhabditis brenneri YIELDED MORE IN CAT TIEN BUT LESS IN CUC PHUONG NATIONAL PARKS
Keywords:
Caenorhabditis elegans, cultivation, diversity of nematodes, DNA barcoding, isolationAbstract
Organisms within subpopulations of the same species may be diverse in respect of any traits because they have life histories under different conditions. We report the research of the diversity of Caenorhabditis brenneri: the isolation of the nematodes from decomposing vegetation collected in Cat Tien, and Cuc Phuong National Parks of Vietnam, molecular classification with DNA barcoding, and cultivation on artificial growth media of the wild-type C. brenneri. We successfully isolated, and cultured 20 wild-type strains of C. brenneri with highly conserved 18S rDNA (99.76% to 100%) to a control species C. brenneri CB5161, and found a likely high diversity amongst them. This result indicated the high potential diversity of the wild-type C. brenneri in Vietnam. We would extend the research of the diversity of C. brenneri and use this species as a model organism in biological studies in the future. We will use the strains of C. brenneri in this research to study the key genetic factors regulating differences within C. brenneri strains.
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