Study on the production of multi-racial microorganism inoculum and evaluating its effective ness for producing Pinus merkusii seedlings in the nursery

Abstract

Pine is a multi-purpose tree, which can generate economic benefits and also
protect the environment. However, there is a variety of diseases that pines can suffer in the nursery such as yellow stunted growth by lack of fungal symbiotic association, damping off by Fusarium oxysporum. Producing multi-racial microorganism inoculum can help stimulate the growth and reduce diseases of Pinus merkusii, meet the demand to produce high quality seedlings for reforestation. Multi-racial microorganism preparation including 35% apatite powder, 35% humus, 5g Pisolithus tinctorius symbiotic fungi, 500ml IAA bacteria solution, 500ml microbes decompose phosphate bacteria solution, 500ml antagonistic bacteria pathogenic fungi bacteria solution, 500ml symbiotic bacteria liquyd nitrogen fixed, 20% Potassium polyacrylamide brought the highest microbial density and activity. The microbial density of the inoculum remained nearly unchanged when stored at room temperature in a period of 6 months. Using 2gr of the inoculum per seedling, after 2 month there was insignificant difference in height and stem diameter. After 4 months, 6 months and 8 months, using the inoculum resulted in outstanding results of height and stem diameter (23% and 28% in height compared to the control formula). Pinus merkusii after 8 months was 22.6cm in height and 4.12mm in stem diameter, had lowest rate of disease and highest rate of symbiotic than any other microbial and control formulas.