Evavaltion of the Rotundin content of  Stephania brachyandra (Diels) gene sources in Thai Nguyen province

Văn Thảo Dương

doi:10.70169/VJFS.919

Từ khóa:

Extraction, l-tetrahydropalmatine, NMR, Rotundin, Stephania brachyandra (Diels)

Tóm tắt

The Stephania brachyandra plant, naturally found in Thai Nguyen, is scientifically known as Stephania brachyandra. It belongs to the genus Stephania Lour., family Menispermaceae, order Menispermaceae. This plant is used as a raw material to extract L-tetrahydropalmatine (the active ingredient known as Rotundin) with the chemical formula C21H25NO4. Rotundin is an alkaloid with sedative, sleep-inducing, and memory-reducing effects. The purpose of this study is to evaluate tof Rotundin content in the Stephania brachyandra species and analyze the correlation between the size of the specíe tubers and the content of Rotudin in Stephania brachyandra naturally distributed in Thai Nguyen province. The analysis results show that the content of Rotundin in 160 samples of naturally distributed Stephania brachyandra in Thai Nguyen province ranges from 90.4 to 981 mg/100g. The analysis of the correlation coefficient between tuber diameter and Rotundin content shows a strong relationship between Rotundin content and tuber diameter. This demonstrates diversity in active ingredient content among natural Stephania brachyandra plant samples, thereby confirming their significant potential as a medicinal resource.

Tài liệu tham khảo

Pham, N. T. T., Le, D. P., Pham, K. T. N., Thipphavong, X., & Chu, M. H. (2021). DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam. Journal of Applied Biology & Biotechnology, 9, 63-70.

Vu, T. C., Bui, H. Q., Choudhary, R. K., Xia, N. H., & Lee, J. (2016). Stephania subpeltata HS Lo (Menispermaceae): A new record for the Flora of Vietnam. Korean Journal of Plant Taxonomy, 46(3), 288-294.

Chinh, V. T., Van, D. N., Tran, V. T., & Xia, N. H. (2019). Stephania polygona (Menispermaceae), a new species from Southern Vietnam. Phytotaxa, 400(3), 211-4.

Hang, N. T., Huy, N. Q., Tung, P. H. T., & Van Thuy, H. (2014). Taxonomy of some species in the genus Stephania Lour. Vietnam using rADN-ITS sequences. Journal of Pharmacy, 54(9), 55-59.

Xie, D., He, J., Huang, J., Xie, H., Wang, Y., Kang, Y., ... & Guo, J. (2015). Molecular phylogeny of Chinese Stephania (Menispermaceae) and reassessment of the subgeneric and sectional classifications. Australian Systematic Botany, 28(4), 246-255.

Chinh, V. T., Van, D. N., Tran, V. T., & Xia, N. H. (2019). Stephania polygona (Menispermaceae), a new species from Southern Vietnam. Phytotaxa, 400(3), 211-4.

Pham, N. T. T., Le, D. P., Pham, K. T. N., Thipphavong, X., & Chu, M. H. (2021). DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam. Journal of Applied Biology & Biotechnology, 9, 63-70.

Nong, T. T. A. (2017). Study on the phytomorphology of the Albizia myriophylla Benth. plants collected in Thai Nguyen, Vietnam. Vietnam J Sci Technol, 17(6), 10-2.

Singh, J., Dilta, B. S., Gupta, Y. C., Sharma, B. P., Baweja, H. S., & Kaushal, S. (2016). Effect of growing media and paclobutrazol on growth, flowering and pot presentability of geranium, Pelargonium x hortorum LH Bailey. International Journal of Farm Sciences, 6(2), 128-136.

Fazekas, A. J., Burgess, K. S., Kesanakurti, P. R., Graham, S. W., Newmaster, S. G., Husband, B. C., ... & Barrett, S. C. (2008). Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PloS one, 3(7), e2802.

Lahaye, R., Van der Bank, M., Bogarin, D., Warner, J., Pupulin, F., Gigot, G., ... & Savolainen, V. (2008). DNA barcoding the floras of biodiversity hotspots. Proceedings of the National Academy of Sciences, 105(8), 2923-2928.

Kang, Y., Deng, Z., Zang, R., & Long, W. (2017). DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests. Scientific reports, 7(1), 12564.

Phong, D. T., Van Tang, D., Hien, V. T. T., Ton, N. D., & Van Hai, N. (2014). Nucleotide diversity of a nuclear and four chloroplast DNA regions in rare tropical wood species of Dalbergia in Vietnam: a DNA barcode identifying utility. Asian Journal of Applied Sciences, 2(2).

Scheunert, A., & Heubl, G. (2014). Diversification of Scrophularia (Scrophulariaceae) in the Western Mediterranean and Macaronesia–Phylogenetic relationships, reticulate evolution and biogeographic patterns. Molecular Phylogenetics and Evolution, 70, 296-313.

Warwick, S. I., Mummenhoff, K., Sauder, C. A., Koch, M. A., & Al-Shehbaz, I. A. (2010). Closing the gaps: phylogenetic relationships in the Brassicaceae based on DNA sequence data of nuclear ribosomal ITS region. Plant Systematics and Evolution, 285, 209-232.