EVALUATING THE INHIBITORY EFFICACY OF BACTERIA STRAINS AGAINST WOOD PATHOGENIC FUNGI
Keywords:
Antifungal bacteria, Bacillus amyloliquefaciens, Chitinophaga varians, wood-damaging fungiAbstract
Characterization of bacterial strains against wood pathogenic fungi is a critical step to evaluate their potential. In this study, the bi-culture and agar diffusion methods were used to evaluate the inhibitory efficacy of 03 bacterial strains isolated in Lang Son province including Chitinophaga varians, Bacillus subtilis and Bacillus amyloliquefaciens against 04 common species of wood-damaging fungi and the influence of some culture conditions on the broth-derived extracellular fluid’s antagonistic activity were also investigated. The results showed that all of 03 bacterial strains had strong antagonistic activity against the fungi Aspergillus niger and Aureobasidium pullulans, and weak antagonistic activity against the fungi Lasiodiplodia theobromae and Trichoderma atroviride. Based on agar diffusion test, Bacillus amyloliquefaciens was preliminarily assessed as the most potential bacterial strain for further research and application, whereas Chitinophaga varians was a new discovery as the role of biocontrol bacterial agent against wood-damaging fungi. The results also showed that adding irons (II) to LB medium reduced the extracellular fluid’s antagonistic activity. The optimal culture duration to obtain antifungal extracellular fluid against Aspergillus niger of Chitinophaga varians, Bacillus subtilis, Bacillus amyloliquefaciens were 3 days, 1 day and 1 day, respectively.
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