In vitro PROPAGATION OF NEW EUCALYPTUS HYBRID CLONES (Eucalyptus urophylla  Eucalyptus grandis) UG105, UG111 AND UG117

Abstract

Study on propagating of new eucalyptus hybrid clones (Eucalyptus urophylla ´  Eucalyptus grandis) UG105, UG111 and UG117 by tissue culture method was investigated. The results contributed to the process of selecting and creating new Eucalyptus hybrids. The sterilization of samples with 0.05% HgCl₂ in 6 minutes provided the highest effective rate of shoots for studied eucalyptus lines. The effective budding rate of UG105, UG111 and UG117 were 56.9%; 55.4% and 54.9%, respectively; while sterilization with 2.5% Javel in 12 minutes archieved 44.0%; 45.1% and 45.3%, respectively. Effective shoots were regenerated in modified Murashige and Skoog medium (MS*) supplemented in MS* + 1 mg/l BAP + 0.5 mg/l NAA+ 0.1 m g/l GA3. Shoot multiplication rate and average shoot length were 2,72 times and 2.96 cm for UG105; 2.90 times and 2.85 cm for UG111; and 2.92 times and 2.89 cm for UG117. The suitable rooting medium for studied clones was 1/2 MS* + 1.5 mg/l IBA + 0.5 mg/l ABT to get the rooting rate of 86.67%; 82.22%; 83.33% respectively and the number of roots/buds were 3.46; 3.42; 3.39 respectively. The acclimatization time for best results was 15 days with the survival rate of 86.89% for UG105; 88.89% for UG111; and 88.67% for UG117, the height reached 5.16 cm; 5.35 cm and 5.29 cm respectively.