SELECTION AND STUDYING OF THE BIOSYNTHETIC ABILITY OF MANGANESE PEROXIDASE (MNP) OF MUSHROOM STRAINS, ORIENTING THE APPLICATION TO DEGRADE LIGNIN
Keywords:
Degrading lignin, increasing production, Manganese peroxidase, Pleurotus sp. PL3, selectionAbstract
Manganese peroxidase (MnP) is an enzyme that is able to degrade lignin and some toxic organic compounds. MnP is biosynthesized by fungi, bacteria and actinomycetes. In this study, the result of the selection of MnP biosynthetic fungal strains and their MnP biosynthetic potential are presented. Research results have selected the fungal strain Pleurotus sp. PL3 with the strongest MnP activity among the 8 investigated strains. MnP biosynthetic activity of Pleurotus sp. PL3 strain reached 2.03 U/mL in PDA medium supplemented with 0.5% NH4NO3, 3% glucose, pH 7.0, temperature culture 30°C for 9 days culture. This research is the basis for the production of MnP enzyme to degrade lignin in the wood processing industry.
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